Dentin slice model of dental stem cells in a fibrin-agarose construct for dental pulp regeneration
Abstract
Objectives: To implement a dentin slice model of mesenchymal stem cells derived from dental tissues in a fibrin-agarose construct for dental pulp regeneration.
Material and Methods: MSCs derived from different oral cavity tissues were combined with a fibrin-agarose construct at standard culture conditions. Cell viability and proliferation tests were assayed using a fluorescent cell dye Calcein/Am and WST-1 kit. The proliferation assay was evaluated at 24, 48, 72, and 96 hours. Also, we assessed the dental pulp stem cells (DPSCs) cell morphology inside the construct with histological stains such as Hematoxylin and Eosin, Masson's trichrome, and Periodic acid–Schiff. In addition, we elaborated a tooth dentin slice model using a culture of DPSC in the fibrin–agarose constructs co-adhered to dentin walls.
Results: The fibrin-agarose construct was a biocompatible material for MSCs derived from dental tissues. It provided good conditions for MSCs' viability and proliferation. DPSCs proliferated better than the other MSCs, but the data did not show significant differences. The morphology of DPSCs inside the construct was like free cells. The dentin slice model was suitable for DPSCs in the fibrin-agarose construct.
Conclusion: Our findings support the dentin slice model for future biological use of fibrin-agarose matrix in combination with DPSCs and their potential use in dental regeneration. The multipotency, high proliferation rates, and easy obtaining of the DPSCs make them an attractive source of MSCs for tissue regeneration.
References
[2]. Liu M, Zeng X, Ma C, Yi H, Ali Z, Mou X, Li S, Deng Y, He N. Injectable hydrogels for cartilage and bone tissue engineering. Bone Res. 2017;5:17014. doi: 10.1038/boneres.2017.14. PMID: 28584674; PMCID: PMC5448314.
[3]. y Baena AR, Casasco A, Monti M. Hypes and Hopes of Stem Cell Therapies in Dentistry: a Review. Stem Cell Rev Rep. doi: 10.1007/s12015-021-10326-4.
[4]. Khorasani HR, Sanchouli M, Mehrani J, Sabour D. Potential of Bone-Marrow-Derived Mesenchymal Stem Cells for Maxillofacial and Periodontal Regeneration: A Narrative Review. Int J Dent. 2021;2021:4759492. doi: 10.1155/2021/4759492. PMID: 34795761; PMCID: PMC8594991.
[5]. Wagenbrenner M, Mayer-Wagner S, Rudert M, Holzapfel BM, Weissenberger M. Combinations of Hydrogels and Mesenchymal Stromal Cells (MSCs) for Cartilage Tissue Engineering-A Review of the Literature. Gels. 2021;7(4):217. doi: 10.3390/gels7040217. PMID: 34842678; PMCID: PMC8628761.
[6]. Angelopoulos I, Brizuela C, Khoury M. Gingival Mesenchymal Stem Cells Outperform Haploidentical Dental Pulp-derived Mesenchymal Stem Cells in Proliferation Rate, Migration Ability, and Angiogenic Potential. Cell Transplant 2018; 27: 967–978.
[7]. Diogenes A, Henry MA, Teixeira FB, Hargreaves KM. An update on clinical regenerative endodontics. Endod Topics. 2013;28(1):2–23. doi: 10.1111/etp.12040
[8]. Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Prockop Dj, Horwitz E. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006;8(4):315-7. doi: 10.1080/14653240600855905. PMID: 16923606.
[9]. Jovic D, Yu Y, Wang D, et al. A Brief Overview of Global Trends in MSC-Based Cell Therapy. Stem Cell Rev Rep. doi: 10.1007/s12015-022-10369-1.
[10]. Eramo S, Natali A, Pinna R, Milia E. Dental pulp regeneration via cell homing. Int Endod J. 2018;51(4):405-419. doi: 10.1111/iej.12868. Epub 2017 Nov 7. PMID: 29047120.
[11]. Inostroza C, Vega-Letter AM, Brizuela C, Castrillón L, Saint Jean N, Duran CM, Carrión F. Mesenchymal Stem Cells Derived from Human Inflamed Dental Pulp Exhibit Impaired Immunomodulatory Capacity In Vitro. J Endod. 2020;46(8):1091-1098.e2. doi: 10.1016/j.joen.2020.05.003. Epub 2020 May 15. PMID: 32422164.
[12]. Liu P, Zhang Y, Ma Y, Tan S, Ren B, Liu S, Dai H, Xu Z. Application of dental pulp stem cells in oral maxillofacial tissue engineering. Int J Med Sci. 2022;19(2):310-320. doi: 10.7150/ijms.68494. PMID: 35165516; PMCID: PMC8795794.
[13]. Strauss FJ, Nasirzade J, Kargarpoor Z, Stähli A, Gruber R. Effect of platelet-rich fibrin on cell proliferation, migration, differentiation, inflammation, and osteoclastogenesis: a systematic review of in vitro studies. Clin Oral Investig. 2020;24(2):569-584. doi: 10.1007/s00784-019-03156-9. Epub 2019 Dec 26. PMID: 31879804; PMCID: PMC6988133.
[14]. Yin W, Qi X, Zhang Y, Sheng J, Xu Z, Tao S, Xie X, Li X, Zhang C. Advantages of pure platelet-rich plasma compared with leukocyte- and platelet-rich plasma in promoting repair of bone defects. J Transl Med. 2016;14:73. doi: 10.1186/s12967-016-0825-9. PMID: 26980293; PMCID: PMC4792107.
[15]. Martín-Piedra MA, Alaminos M, Fernández-Valadés-Gámez R, España-López A, Liceras-Liceras E, Sánchez-Montesinos I, Martínez-Plaza A, Sánchez-Quevedo MC, Fernández-Valadés R, Garzón I. Development of a multilayered palate substitute in rabbits: a histochemical ex vivo and in vivo analysis. Histochem Cell Biol. 2017;147(3):377-388. doi: 10.1007/s00418-016-1489-5. Epub 2016 Sep 6. PMID: 27600719.
[16.] Carriel V, Garrido-Gómez J, Hernández-Cortés P, Garzón I, García-García S, Sáez-Moreno JA, Del Carmen Sánchez-Quevedo M, Campos A, Alaminos M. Combination of fibrin-agarose hydrogels and adipose-derived mesenchymal stem cells for peripheral nerve regeneration. J Neural Eng. 2013;10(2):026022. doi: 10.1088/1741-2560/10/2/026022. Epub 2013 Mar 26. PMID: 23528562.
[17]. Trope M. Treatment of the immature tooth with a non-vital pulp and apical periodontitis. Dent Clin North Am 2010; 54: 313–324.
[18]. Zhang S, Zhang W, Li Y, Ren L, Deng H, Yin X, Gao X, Pan S, Niu Y. Human Umbilical Cord Mesenchymal Stem Cell Differentiation Into Odontoblast-Like Cells and Endothelial Cells: A Potential Cell Source for Dental Pulp Tissue Engineering. Front Physiol. 2020;11:593. doi: 10.3389/fphys.2020.00593. PMID: 32714196; PMCID: PMC7344301.
[19]. Huang Z, Newcomb CJ, Bringas P Jr, Stupp SI, Snead ML. Biological synthesis of tooth enamel instructed by an artificial matrix. Biomaterials. 2010;31(35):9202-11. doi: 10.1016/j.biomaterials.2010.08.013. Epub 2010 Sep 24. PMID: 20869764; PMCID: PMC2956865.
[20]. Soares DG, Bordini EAF, Swanson WB, de Souza Costa CA, Bottino MC. Platform technologies for regenerative endodontics from multifunctional biomaterials to tooth-on-a-chip strategies. Clin Oral Investig. 2021;25(8):4749-4779. doi: 10.1007/s00784-021-04013-4. Epub 2021 Jun 28. PMID: 34181097; PMCID: PMC8546585.
[21]. Tavares S, Pintor A, Mourão CFAB, Magno M, Montemezzi P, Sacco R, Alves G, Scelza MZ. Effect of Different Root Canal Irrigant Solutions on the Release of Dentin-Growth Factors: A Systematic Review and Meta-Analysis. Materials (Basel). 2021;14(19):5829. doi: 10.3390/ma14195829. PMID: 34640224; PMCID: PMC8510123.
[22]. Bottino MC, Pankajakshan D, Nör JE. Advanced Scaffolds for Dental Pulp and Periodontal Regeneration. Dent Clin North Am 2017; 61: 689–711.
[23]. Martin-Piedra MA, Gironés-Camarasa B, España-López A, Fernández-Valadés Gámez R, Blanco-Elices C, Garzón I, Alaminos M, Fernández-Valadés R. Usefulness of a Nanostructured Fibrin-Agarose Bone Substitute in a Model of Severely Critical Mandible Bone Defect. Polymers (Basel). 2021;13(22):3939. doi: 10.3390/polym13223939. PMID: 34833238; PMCID: PMC8618832.
[24]. Andrade Aldana C, Ugarte Amenabar F, Inostroza Silva C, Diaz Calderon P, Rosenberg Messina D, Pinto Carrasco N, Quirynen M. The impact of gender and peripheral blood parameters on the characteristics of L-PRF membranes. J Oral Biol Craniofac Res. 2022;12(6):753-759. doi: 10.1016/j.jobcr.2022.08.020. Epub 2022 Sep 1. PMID: 36118143; PMCID: PMC9478449.
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