In vitro bacterial adherence on teeth submitted to whitening with Musa paradisiaca.
Abstract
Objetive: To compare in vitro bacterial adherence on teeth submitted to whitening with 50% ethanolic extract of Musa paradisiaca and 35% hydrogen peroxide.
Material and Methods: The study was experimental and used 18 premolars that were grouped into: G1 (control), G2 (50% ethanol extract of Musa paradisiaca) and G3 (35% hydrogen peroxide). The teeth were then exposed to a Streptococcus mutans culture for 24 hours, followed by centrifugation in thioglycolate broth. A culture on trypticase soy agar was done with a 1 in 100 dilution, and after 48 hours colony forming units (CFU) were counted. Statistical analysis was performed using the ANOVA test, complemented by the Bonferroni post-hoc.
Results: Bacterial adherence was 77x105 CFU/ml in Group 3 using 35% hydrogen peroxide, 40x105 CFU/ml in Group 2 using 50% ethanol extract of Musa paradisiaca, and 89x104 CFU/ml in Group 1 (control). The difference between the three groups was significant (p=0.000).
Conclusion: Both whitening methods cause bacterial adherence to the tooth surface, although to a lower degree with Musa paradisiaca.eses.
References
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[30]. Asmawati A, Thalib B, Natsir N, Rieuwpassa I E, Mahardhika A, Hasyim R. An analysis of the compounds of dental enamel after application strawberry gel with energy-dispersive. J Int Dent Med Res. 2018; 11(2):656-662.
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[37]. Yudhit A. Potency of banana (Musa paradisiaca var. Raja) peel extract as color changes agent of human teeth ( In-Vitro ). IOSR J Dent Med Sci. 2019; 18(10): 68-71. doi:10.9790/0853-1810056871
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[41]. Sultan R, Shawkkat M, Hadi S. Antimicrobial, antibiofilm and antiplasmid activity of fruit peel extracts on bacterial dental caries. Curr Res Microbiol Biotechnol. 2017; 5(5): 1266-1272.
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[46]. Vishwakarma P, Karale R, Srirekha A, Hedge J, Savitha B, Srinivasan A. The effect of home bleaching agents on the surface roughness and fracture toughness of composite resin materials. Dentistry. 2014; 4(7):246. doi: 10.4172/2161-1122.1000246 .
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[2]. Zavanelli AC, Sônego MV, Zavanelli RA, Mazaro JVQ, Falcón- Antenucci RM . Perception and expectation. What do patients really want from the dental treatment ? Rev Gauch odontol. 2017; 65(3):243-248. doi : 10.1590/1981-863720170002000093257 .
[3]. Silva FBD, Chisini LA, Demarco FF, Horta BL, Correa MB. Desire for tooth bleaching and treatment performed in Brazilian adults: findings from a birth cohort. Arm Oral Res. 2018; 32:e12. doi.rog/10.1590/1807-3107bor-2018.vol32.0012
[4]. Epple M, Meyer F, Enax J. A critical review of modern concepts for tooth whitening. Dent J. 2019; 7(3):79. doi:10.3390/dj7030079 .
[5]. Vargas - Koudriavtsev T, Durán-Sedó R, Sáenz-Bonilla P, Bonilla-Mora V, Guevara- Bertsch M, Jiménez-Corrales RA, Herrera-Sancho OA. Effects of tooth whitening agents on phosphate concentration in tooth enamel by means of Raman spectroscopy. Rev odontol Mex. 2015; 19(4):232-39. doi:10.1016/j.rodmex.2015.10.004
[6]. Féliz -Matos L, Hernández LM, Abreu N. Dental bleaching techniques; hydrogen-carbamide peroxides and light sources activation, an update: mini review article. Open Dent J. 2014; 8(1):264-268. doi: 10.2174/1874210601408010264
[7]. Molina BN, Genaro LE, Fazanaro MCS, Ohata G, Dantas AAR. Efficacy and adverse effects of dental bleaching in the office: literature review. Biomed J Sci & Tech Res. 2021 Apr 28; 35(3):27628-27636. doi : 10.26717/BJSTR.2021.35.005694
[8]. Hurd A, Palacios J, Pallotini G, González JM, Maláver P, López C. Effect of tooth whitening with hydrogen peroxide at different concentrations on the enamel surface: an in vitro study . J Odont Col. 2015; 8(16):8-30.
[9]. Fatima N. In vitro comparative study of in-office and home bleaching agents on surface micro-morphology. J Coll of Physicians Surg Pak. 2016; 26(1):9-12.
[10]. Presoto CD, Bortolatto JF, de Carvalho PPF, Trevisan TC, Floros MC, Junior OBO. New parameter for in-office dental bleaching. Case Rep Dent. 2016; 2016(1): 6034757. doi: 10.1155/2016/6034757
[11]. Abouelfotouh IFI, Fahmy OMI, Khairy AE, Eldine DMS. A comparative study of different bleaching techniques, regarding the color change, stability and postoperative hypersensitivity: a randomized controlled clinical trial. Stomatology Dis Sci. 2018 ; 2(5):5. doi:10.20517/2573-0002.2018.01
[12]. Llena C, Esteve I, Forner L. Effect of hydrogen and carbamide peroxide in bleaching, enamel morphology, and mineral composition: in vitro study. J Contemp Dent Pract . 2017; 18(7):576-582. doi: 10.5005/jp-journals-10024-2087
[13]. Lekshmi J, Nair SS, Pillai R, Varghese NO, Salim AA, Murali N. Spectrophotometric analysis of color changes in enamel following exposure to herbal bleaching agents: an in vitro study. Cons Dent Endod J. 2018; 3(2):62-66. doi:10.5005/jp-journals-10048-0041
[14]. Zheng CY, Pan J, Wang ZH, Wang Y. Effects of hydrogen peroxide-containing bleaching on the growth of Streptococcus mutans biofilm on enamel disc surface. J Peking Univ , Health sci. 2014; 46(1):30-34. doi:10.3969/j.issn.1671-167X.2014.01.006
[15]. Ittatirut S, Matangkasombut O, Thanyasrisung P. In-office bleaching gel with 35% hydrogen peroxide enhanced biofilm formation of early colonizing streptococci on human enamel. J Dent. 2014; 42(11):1480-6. doi:10.1016/j.jdent.2014.08.003
[16]. Al-Jubori SH, Al-Sabawi NA, Taha MY. Study of the adherence of Streptococcus mutans on bleached and fluoridated tooth surfaces (an in vitro study). Al –Rafidain Dent J. 2013; 13(1):116-121. doi:10.33899/order.2012.84688
[17]. Romero AJM, Romero AC. Streptococcus adhesion mutans in human permanent teeth subjected to two bleaching agents. Kiru . 2009; 6(1):39-45.
[18]. Hosoya N, Honda K, Iino F, Arai T. Changes in enamel surface roughness and adhesion of Streptococcus mutans to enamel after vital bleaching. J Dent. 2003; 31(8):543-548. doi:10.1016/S0300-5712(03)00109-X
[19]. Kalliath C, Mukunda A, Pynadath M, Venugopal V, Prethweeraj J. Comparison between the effect of commercially chemical teeth whitening paste and teeth whitening containing ingredients of herbal origin on human enamel. Ayu. 2018; 39(2): 113-117.
[20]. Lee JH, Bae SS. A study on the whitening effect of fruit extracts and the changes in the components of the teeth. Indian J Sci Technol , 2016; 9S( 1):1-7. doi:10.17485/ ijst /2016/v9iS1/109826
[21]. Rahmawan D T, Wijayaningrum KS, Puspita S. Comparison of immersion time between strawberry (Fragaria x ananassa) juice and 35% carbamide peroxide on tooth discoloration. MMJKK. 2018; 18(1):20-4. doi:10.18196/mm.180110
[22]. Kwon SR, Kurti SR, Oyoyo U, Li Y. Effect of various tooth whitening modalities on microhardness, surface rough-ness and surface morphology of the enamel. Dentistry. 2015; 103(3):274-279. doi:10.1007/s10266-014-0163-4
[23]. Neha R, Priya VV, Arthanari A, Gayathri R, Kavithats , Reshma PK. Effect of strawberry extract on tooth discoloration and morphology – An in vitro study. J Pharm Rest Int. 2022; 34(5B):27-36. doi:10.9734/JPRI/2022/v34i5B35412 .
[24]. Radhakrishnan R, Nesamani R, Sanjeev K, Sekar M. Effect of bleaching with strawberry extract and hydrogen peroxide on color change, surface morphology and micro-shear bond strength of resin composite to enamel surface. J Clin Diagnostic Res.2021; 15(3): ZC01-ZC06. doi : 10.7860/JCDR/2021/47070.14601 .
[25] Renatami RK, Noerdin A, Irawan B, Soufyan A. Effects of different concentrations of bilimbi (Averrhoa bilimbi L.) extract gel on enamel surface roughness. J Phys Conf Ser. 2018; 1073(3):0332012. doi:10.1088/1742-6596/1073/3/032012 .
[26]. Karima F, Eriwati YK, Triaminingsih S. Effect of belimbing wuluh ( Averrhoa bilimbi L.) extract gel exposure duration to surface roughness of enamel. J Phys Conf Ser. 2017; 884 (1): 012071. doi : https://doi.org/10.1088/1742-6596/884/1/012071 .
[27]. Hartanto A, Rianti D, Meizarini A. Strawberry paste application as bleaching materials towards color change and enamel surface microhardness. JMKG. 2012; 1(1):7-14.
[28]. Asmawati A, Rieuwpassa IE. Comparison of enamel hardness after the application of dental bleaching agents strawberry gel and 10% carbamide peroxide. J Dentomaxillofac Sci. 2018; 3(1):17-19. doi:10.15562/jdmfs.v3i1.626
[29]. Dewi D D , Mozartha M, Bikarindrasari R. Effect of apple gel extract application ( Malus domestica ) on dental calcium solubility. Denta J Ked Gigi. 2019; 13(2):16-23.
[30]. Asmawati A, Thalib B, Natsir N, Rieuwpassa I E, Mahardhika A, Hasyim R. An analysis of the compounds of dental enamel after application strawberry gel with energy-dispersive. J Int Dent Med Res. 2018; 11(2):656-662.
[31]. Kwon SR, Meharry MR, Oyoyo U, Li Y. Efficacy of do-it-yourself whitening as compared to conventional tooth whitening modalities: an in vitro study. Opera Dent. 2015; 40(1):E21-E27. doi :10.2341/13-333-LR
[32]. Naikwade PV, Gaurav S, Sharayu D, Kailas J. Evaluation of antibacterial properties of Musa paradisiaca L. leaves . Bio Disc. 2015; 6(1-I): 80-4.
[33]. Lakshmi V, Agarwal SK, Mahdi AA. An Overview of Musa paradisiaca Linn. NPAIJ. 2015; 11(4):105-9.
[34]. Barua N, Das M. An Overview of pharmacological activities of Musa sapientum and Musa paradisiaca. Int J Pharmacognosy and Phytochem Rest. 2013; 4(2): 852-858 .
[35]. Juárez K. Comparative study between natural homemade bleaching agents and bleaching agents applied in the dental office to determine their effectiveness, period March-July 2016. [Bachelor's degree]. store; 2016.
[36]. Borja F. Efficacy of banana peel as tooth whitening agent in in vitro premolars : Universidad Alas Peruanas, Arequipa - 2013. [Bachelor's degree]. Arequipa: 2014.
[37]. Yudhit A. Potency of banana (Musa paradisiaca var. Raja) peel extract as color changes agent of human teeth ( In-Vitro ). IOSR J Dent Med Sci. 2019; 18(10): 68-71. doi:10.9790/0853-1810056871
[38]. Gutierrez AJ, Turco WR. Effectiveness of Musa paradisiaca husk as a bleaching agent in the chromatic change of bovine teeth. [Bachelor's degree]. Huancayo: 2020.
[39]. Quispe RI, Soto KJ, Tipiana RR. Banana peel as a homemade tooth whitener. Ika: 2017.
[40]. Imam MZ, Akter S. Musa paradisiaca L. and Musa sapientum L.: A phytochemical and pharmacological review. J App Pharm Sci 2011; 1:14-20.
[41]. Sultan R, Shawkkat M, Hadi S. Antimicrobial, antibiofilm and antiplasmid activity of fruit peel extracts on bacterial dental caries. Curr Res Microbiol Biotechnol. 2017; 5(5): 1266-1272.
[42]. Septian R. Efektivitask kandungan antibacterial ekstrak kulit pisang kepok ( Musa Paradisiaca L.) pada berbagai consentrasi dalam menghambat pertumbuhan bacteria Streptococcus mutans penyebab karies gigi. [ Doctorate ]. Surabaya: 2019.
[43]. Behiry SI, Olka MK, Alamri SA, EL- Hefny M, Salem MZM, Alaraidh IA, Ali HM, Al -Ghtani SM, Monroy JC, Salem AZM. Antifungal and antibacterial activities of Musa paradisiaca L. peel extract: HPLC analysis of phenolic and flavonoid contents. processes. 2019 Apr 15; 7 (215): 1-11. doi : 10.3390/pr7040215 .
[44]. Centers for Disease Control and Prevention – The National Institutes of Health (CDC-NIH). Biosafety in Microbiology and Biomedicine Laboratories. 4th ed. Atlanta: Public Health and Safety Office; 1999.
[45]. Puspasari N, Effendi C, Nugraeni Y. Effect of apple juice on whitening teeth after immersion in coffee solution in vitro. IDJ. 2012; 1(2): 17-19. doi:10.18196/di.v1i2.526 .
[46]. Vishwakarma P, Karale R, Srirekha A, Hedge J, Savitha B, Srinivasan A. The effect of home bleaching agents on the surface roughness and fracture toughness of composite resin materials. Dentistry. 2014; 4(7):246. doi: 10.4172/2161-1122.1000246 .
[47]. Attia RM, Kamel MM. Changes in Surface roughness of bleached enamel by using different remineralizing agents. Tanta Dent J. 2016; 13(4):179-186. doi:10.4103/1687-8574.195707 .
[48]. Chabuck ZAG, Al -Charrakh AH, Hindi NKK, Hindi SKK. Antimicrobial effect of aqueous banana peel extract, Iraq. Res Gate: Pharm Sci. 2013; 1:73-75.
[49]. Aquino CF, Salomao LCC, Ribeiro SMR, de Siqueira DL, Cecon PR. Carbohydrates, phenolic compounds and antioxidant activity in pulp and peel of 15 banana cultivars. Rev Bras Frutic . 2016; 38(4):e-090. doi: 10.1590/0100-29452016090 .
[50]. Chandarju S, Mythily R, Kumar CSC. Extraction, isolation and identification of sugars from banana peels by HPLC coupled LC/MS instrument and TLC analysis. J Chem Pharm Res. 2011; 3(3):312-321.
[51]. Mohsien RA. Antimicrobial effect of banana peel and lemon fruit juice extracts. EJBPS. 2017; 4(7): 17-21.
Published
2022-06-30
How to Cite
PANTIGOZO-MORÁN, Úrsula; GONZÁLEZ-CABEZA, José; ESPINOZA-SALCEDO, María.
In vitro bacterial adherence on teeth submitted to whitening with Musa paradisiaca..
Journal of Oral Research, [S.l.], v. 11, n. 3, p. 1-11, june 2022.
ISSN 0719-2479.
Available at: <https://joralres.com/index.php/JOralRes/article/view/joralres.2022.039>. Date accessed: 27 apr. 2024.
doi: https://doi.org/10.17126/joralres.2022.039.
Section
Articles
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