Prevalence of HPV and EBV infection and their relationship with the p53 and PCNA expression in oral carcinoma patients.

  • Dayahindara Veitía Laboratorio de Genética Molecular, Instituto de Oncología y Hematología-MPPS, Caracas.
  • Juan Liuzzi Servicio de cabeza y cuello del Hospital Oncológico ‘Padre Machado’-IVSS, Caracas.
  • Maira Àvila Servicio de cabeza y cuello del Hospital Oncológico ‘Padre Machado’-IVSS, Caracas.
  • Zoraya De Guglielmo Laboratorio de Genética Molecular, Instituto de Oncología y Hematología-MPPS, Caracas.
  • María Correnti Instituto de Investigaciones Odontológicas Raúl Vincentelli, Facultad de Odontología de la UCV, Caracas.

Abstract

Introduction: Infection caused by potentially oncogenic viruses, such as HPV and EBV, favors the role of certain oncoproteins that can induce dysplasias and malignant lesions. Objective: To evaluate the prevalence of HPV and EBV and their relation with the expression of p53 and PCNA in patients with oral carcinoma. Methodology: Twenty-seven oral squamous cell carcinomas (OSCC) were evaluated; DNA extraction was conducted using the QIAamp DNA mini kit; viral detection was obtained using the INNO-LiPA kit for HPV, and nested PCR was used for EBV. The evaluation of molecular markers was performed through immunohistochemical staining. Results: The mean age of the patients was 60.55±13.94 years, and 52% of these were female. Of the patients, 59% were tobacco users and 63% were alcohol consumers. HPV was detected in 70% of the patients with the predominance of genotype 16 (60%). As for EBV infection, it was observed in 59% of cases. p53 and PCNA immunopositivity corresponded to 44% and 59%, respectively. The tongue was the anatomical location with highest positivity for both viruses as well as for the expression of molecular markers. The 48% of the cases presented infection by both viruses. Conclusion: HPV and EBV infection together with the expression of p53 and PCNA were more frequently observed in advanced stages of the disease, suggesting a more relevant role in the progression than in tumor genesis.

Author Biography

Dayahindara Veitía, Laboratorio de Genética Molecular, Instituto de Oncología y Hematología-MPPS, Caracas.

Editor de Journal of Oral Research.

Asesor en Metodología de la Investigación.

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Published
2017-04-25
How to Cite
VEITÍA, Dayahindara et al. Prevalence of HPV and EBV infection and their relationship with the p53 and PCNA expression in oral carcinoma patients.. Journal of Oral Research, [S.l.], v. 6, n. 4, p. 86-91, apr. 2017. ISSN 0719-2479. Available at: <https://joralres.com/index.php/JOralRes/article/view/joralres.2017.026>. Date accessed: 29 mar. 2024. doi: https://doi.org/10.17126/joralres.2017.026.
Section
Articles

Keywords

Carcinoma; VPH; VEB; p53; PCNA.