Effect of Adding Fluoride to Camellia sinensis Against Dentin Erosion Generated by Non-Bacterial Acids

Ricardo Reyes-Mansilla; Donald Ramos-Perfecto; Luis Maita-Véliz; Eduardo López-Pagan; Luis Maita-Castañeda

doi:10.17126/joralres.2024.017

Ricardo Reyes-Mansilla Facultad de Odontología, Universidad Nacional Mayor de San Marcos, Lima, Perú. http://orcid.org/0000-0003-2506-2593
Donald Ramos-Perfecto Facultad de Odontología, Universidad Nacional Mayor de San Marcos, Lima, Perú. http://orcid.org/0000-0001-5870-3962
Luis Maita-Véliz Facultad de Odontología, Universidad Nacional Mayor de San Marcos, Lima, Perú. http://orcid.org/0000-0003-0672-9356
Eduardo López-Pagan Facultad de Odontología, Universidad Nacional Daniel Alcides Carrión, Pasco, Perú. http://orcid.org/0000-0002-3997-0050
Luis Maita-Castañeda Facultad de Odontología, Universidad Nacional Mayor de San Marcos, Lima, Perú. http://orcid.org/0000-0002-0548-0491

DOI: https://doi.org/10.17126/joralres.2024.017

Abstract

Introduction: Natural products are an option to be used in different conditions in the oral cavity, such as Camellia sinensis, which due to its different properties would be beneficial in the erosion of the dental surface.
Objetive: Determinar el efecto del extracto de Camellia sinensis (C. sinensis) adicionado con flúor frente a la acción erosiva de ácidos no bacterianos en la superficie de la dentina humana
Materials and Methods: Quasi-experimental, prospective, longitudinal, and in vitro study, consisting of 50 samples of human dentin, which were classified into 5 study groups: distilled water, hydrochloric acid, 2% C. sinensis extract, sodium fluoride. 2% and 2% C. sinensis extract added with 2% sodium fluoride. The surface roughness of each sample was evaluated with the SRT6200 digital roughness meter, obtaining a total average roughness and the measurements were carried out in two moments.
Results: It was observed that the 2% C. sinensis extract, the 2% sodium fluoride, and the C. sinensis extract added with 2% sodium fluoride, showed a variation in surface roughness between before and after, which was not significant (p<0.05) in all cases. It should be noted that the C. sinensis plus sodium fluoride group is the one that obtained the best variation in the mean surface roughness than the other groups studied.
Conclusions: The 2% C. sinensis extract, 2% sodium fluoride, and the combination of both compounds demonstrated an inhibitory effect against the erosive action of hydrochloric acid (0.01 M) on the dentin surface, not presenting a statistically significant difference in the results.
Keywords: Camellia sinensis; Tooth erosion; Fluorides; Dentina; Surface properties; Prospective studies.

References

1. Montoya C, Ossa E. Composición química y microestructura de la dentina de pacientes colombianos. Rev. Colomb. Mater. 2014;14(5):73-8. doi: 10.17533/udea.rcm.19425

2. Monterde M, Delgado J, Rico M, Gúzman C, Mejía M. Desmineralización-remineralización del esmalte dental. Rev ADM. 2002;59(6):220-2.

3. Soares P, Grippo J. Lesiones cervicales no cariosas e hipersensibilidad cervical en dentina: Etiología, diagnóstico y tratamiento. 1. Ed. Illinois: Grippo J editor; 2017.

4. Imfeld T. Dental erosion. Definition, classification and links. Eur J Oral Sci. 1996;104(1):151-55. doi: 10.1111/j.1600-0722.1996.tb00063.x

5. Peumans M, Politano G, Van Meerbeek B. Tratamiento de lesiones cervicales no cariadas: cuándo, porqué y cómo. Int J. Esthet Dent. 2020; 15(1):8-35.

6. Passos V, Melo M, Lima J, Marçal F, Costa C, Rodrigues L. Active compounds and derivatives of Camellia sinensis responding to erosive attacks on dentin. Braz. Oral Res. 2018;32(1):e40. doi: 10.1590/1807-3107bor-2018.vol32.0040.

7. Reyes-Mansilla R, Cuentas-Robles A, Ramos-Perfecto D. Camellia sinensis, a natural product to support the treatment of medical and stomatological conditions. J Oral Res. 2023;13(1):24-34. doi.org/10.17126/joralres.2023.003

8. Fizikova A, Subcheva E, Kozlov N, Tvorogova V, Samarina L, Lutova L, Khlestkina E. Agrobacterium Transformation of Tea Plants (Camellia sinensis (L.) KUNTZE): A Small Experiment with Great Prospects. Plants (Basel). 2024;13(5):675. doi: 10.3390/plants13050675. PMID: 38475520; PMCID: PMC10934914.

9. Li Y, Wang X, Ban Q, Zhu X, Jiang C, Wei C, Bennetzen JL. Comparative transcriptomic analysis reveals gene expression associated with cold adaptation in the tea plant Camellia sinensis. BMC Genomics. 2019;20(1):624. doi: 10.1186/s12864-019-5988-3. PMID: 31366321; PMCID: PMC6670155.

10. Menezes J, Borba G, Oliveira F, Almeida M, Rodrigues A, Machado A. Volatile compounds and quality analysis in commercial medicinal plants of Camellia sinensis. Cienc Rural. 2019;49(3):e20180548. doi: 10.1590/0103-8478cr20180548

11. Yu Z, Liao Y, Zeng L, Dong F, Watanabe N, Yang Z. Transformation of catechins into theaflavins by upregulation of CsPPO3 in preharvest tea (Camellia sinensis) leaves exposed to shading treatment. Food Res Int. 2019;129(1):1. doi: 10.1016/j.foodres.2019.108842

12. Ni T, Moon N, San O. Pollen morphology, phytochemical test and antimicrobial activities of tea leaves found in wan saing village, Kyaing Tong. J. Myanmar Acad. Arts Sci. 2020; 18(4B): 85-95.

13. Mulugeta G. Effect of Different Shade Tree Species on the Growth and Yield of China Hybrid Tea (Camellia sinensis (L.) Kuntze) at Palampur Tea Research Station, H.P. India. J Nat Sci Res. 2017; 7(4): 15-22.

14. Ahmeda, A, Zangeneh, A, Zangeneh, M. Green synthesis and chemical characterization of gold nanoparticle synthesized using Camellia sinensis leaf aqueous extract for the treatment of acute myeloid leukemia in comparison to daunorubicin in a leukemic mouse model. Appl Organomet Chem. 2020; 34(3): 1474-1504.

15. Jordá N, Picó J. Caracterización y valoración de metabolitos secundarios activos de Té Pu-Erh (Camellia sinensis var. assamica) en diferentes preparados comerciales. [Tesis]. Facultad de Farmacia de la Universidad Miguel Hernández (UMH) en el Campus de San Juan de Alicante. España. 2019.

16. Suyama E, Tamura T, Ozawa T, Suzuki A, Iijima Y, Saito T. Remineralization and acid resistance of enamel lesions after chewing gum containing fluoride extracted from green tea. Aust Dent J. 2011 Dec;56(4):394-400. doi: 10.1111/j.1834-7819.2011.01359.x. Epub 2011 Oct 3. PMID: 22126349.

17. Buzalaf M, Kato M, Hannas A. The Role of Matrix Metalloproteinases in Dental erosion. Adv Dent Res. 2012; 24(2):72-6. doi: 10.1177/0022034512455029.

18. Prado S, Araiza M, Valenzuela E. Eficiencia in vitro de compuestos fluorados en la remineralización de lesiones cariosas del esmalte bajo condiciones cíclicas de pH. Rev Odont Mex. 2014; 18(2):96-104.

19. Zhu J, Pan J, Nong S, Ma Y, Xing A, Zhu X, Wen B, Fang W, Wang Y. Transcriptome Analysis Reveals the Mechanism of Fluoride Treatment Affecting Biochemical Components in Camellia sinensis. Int J Mol Sci. 2019;20(2):237. doi: 10.3390/ijms20020237. PMID: 30634430; PMCID: PMC6359021.

20. Chen D, Chen G, Sun Y, Zeng X, Ye H. Physiological genetics, chemical composition, health benefits and toxicology of tea (Camellia sinensis L.) flower: A review. Food Res Int. 2020;137:109584. doi: 10.1016/j.foodres.2020.109584. Epub 2020 Jul 24. PMID: 33233193.

21. Magalhães AC, Wiegand A, Rios D, Hannas A, Attin T, Buzalaf MA. Chlorhexidine and green tea extract reduce dentin erosion and abrasion in situ. J Dent. 2009;37(12):994-8. doi: 10.1016/j.jdent.2009.08.007. Epub 2009 Sep 3. PMID: 19733206.

22. Oh JW, Muthu M, Pushparaj SSC, Gopal J. Anticancer Therapeutic Effects of Green Tea Catechins (GTCs) When Integrated with Antioxidant Natural Components. Molecules. 2023;28(5):2151. doi: 10.3390/molecules28052151. PMID: 36903395; PMCID: PMC10004647.