Gene expression of collagen type I alpha 2 and its relationship with dental fluorosis.

  • Bertha Gastelbondo-Pastrana Prometeus & Biomedicine Research Group applied to Clinical Sciences, Universidad de Cartagena, Colombia. http://orcid.org/0000-0002-6852-9758
  • Meisser Madera-Anaya Prometeus & Biomedicine Research Group applied to Clinical Sciences, Universidad de Cartagena, Colombia. Research Department, School of Dentistry, Universidad de Cartagena, Colombia. http://orcid.org/0000-0002-2350-4194
  • Amileth Suárez-Causado Prometeus & Biomedicine Research Group applied to Clinical Sciences, Universidad de Cartagena, Colombia. Department of Basic Sciences, Biochemistry Unit, School of Medicine, Universidad de Cartagena, Colombia.

Abstract

Objective: To compare the gene expression levels of collagen type I alpha 2 (COL1A2) in children with and without dental fluorosis. Methods: Cross-sectional study involving 92 children between 5 and 12 years of age. Socio-demographic characteristics, the presence of dental fluorosis by means of the Thylstrup-Fejerskov index, and gene expression analysis of COL1A2 in peripheral blood samples by reverse transcription polymerase chain reaction (RT-PCR) assays, were described. For the descriptive analysis, measures of central tendency, dispersion and proportions were used. Differences between the groups (p<0.05) were established by the Student t-test. Results: Mean age was 8.6 (SD=1.9) years, 51.1% were female; 54 children were diagnosed with fluorosis and 38 without fluorosis; prevalence of dental fluorosis was 58.7% (95% CI: 48.4% -68.9%). Gene expression of COL1A2 was statistically significantly lower (p<0.05) in the participants with dental fluorosis. Conclusion: There are differences in the expression levels of the COL1A2 gene among the population under study. Therefore, COL1A2 may be potentially involved in the development of dental fluorosis.

References

1. Amaral SL, Azevedo LB, Buzalaf MAR, Fabricio MF, Fernandes MS, Valentine RA, Maguire A, Zohoori FV. Effect of chronic exercise on fluoride metabolism in fluorosis-susceptible mice exposed to high fluoride. Sci Rep. 2018;8(1):3211.
2. Arrieta-Vergara KM, González-Martínez F, Luna-Ricardo L. Exploración del riesgo para fluorosis dental en niños de las clínicas odontológicas universidad de Cartagena. Rev Salud Públ. 2011;13(4):672–83.
3. Meng H, Zhang T, Liu W, Wang H, Wang C, Zhao Z, Liu N, Wang W. Sodium fluoride induces apoptosis through the downregulation of hypoxia-inducible factor-1α in primary cultured rat chondrocytes. Int J Mol Med. 2014;33(2):351–8.
4. Huang H, Ba Y, Cui L, Cheng X, Zhu J, Zhang Y, Yan P, Zhu C, Kilfoy B, Zhang Y. COL1A2 gene polymorphisms (Pvu II and Rsa I), serum calciotropic hormone levels, and dental fluorosis. Community Dent Oral Epidemiol. 2008;36(6):517–22.
5. Dawson DV. Preliminary evidence of an association between COL1A2 polymorphisms and dental fluorosis in a population with high fluoride exposure. J Evid Based Dent Pract. 2010;10(2):96–8.
6. Escobar-García D, Mejía-Saavedra J, Jarquín-Yáñez L, Molina-Frechero N, Pozos-Guillén A. Collagenase 1A2 (COL1A2) gene A/C polymorphism in relation to severity of dental fluorosis. Community Dent Oral Epidemiol. 2016;44(2):162–8.
7. Yan X, Hao X, Nie Q, Feng C, Wang H, Sun Z, Niu R, Wang J.
Effects of fluoride on the ultrastructure and expression of Type I collagen in rat hard tissue. Chemosphere. 2015;128:36-41.
8. Pragya G, Vandana KL. Collagen 1 alpha 2 gene polymorphism and dental fluorosis: A polymerase chain reaction study. SRM J Res Dent Sci. 2018;9:6–12.
9. Riksen EA, Landin MA, Reppe S, Nakamura Y, Lyngstadaas SP, Reseland JE. Enamel Matrix Derivative Promote Primary Human Pulp Cell Differentiation and Mineralization. Int J Mol Sci. 2014;15(5):7731–49.
10. Zhang YL, Luo Q, Deng Q, Li T, Li Y, Zhang ZL, Zhong JJ. Genes associated with sodium fluoride-induced human osteoblast apoptosis. Int J Clin Exp Med. 2015;8(8):13171–8.
11. Pramanik S, Saha D. The genetic influence in fluorosis. Environ Toxicol Pharmacol. 2017;56:157–62.
Published
2018-07-30
How to Cite
GASTELBONDO-PASTRANA, Bertha; MADERA-ANAYA, Meisser; SUÁREZ-CAUSADO, Amileth. Gene expression of collagen type I alpha 2 and its relationship with dental fluorosis.. Journal of Oral Research, [S.l.], v. 7, n. 6, p. 232-235, july 2018. ISSN 0719-2479. Available at: <https://joralres.com/index.php/JOralRes/article/view/joralres.2018.056>. Date accessed: 29 mar. 2024. doi: https://doi.org/10.17126/joralres.2018.056.
Section
Communications

Keywords

dental fluorosis; dental enamel; gene expression; collagen.