Antifungal Activity of the Essential Oil of Cymbopogon citratus (DC) Stapf. An in vitro study.

  • Naiana Braga da Silva State University of Paraiba, Campina Grande.
  • Marianne de Lucena Rangel Federal University of Paraiba, Joao Pessoa.
  • Bruno Barbosa Almeida State University of Paraiba, Campina Grande.
  • Ricardo Dias de Castro Federal University of Paraiba, Joao Pessoa.
  • Ana Maria Gondim Valença Federal University of Paraiba, Joao Pessoa.
  • Alessandro Leite Cavalcanti State University of Paraiba, Campina Grande.

Abstract

Abstract: Aim. To evaluate the antifungal potential of the essential oil of Cymbopogon citratus by determining the Minimum Inhibitory Concentration (MIC) and the Minimum Fungicidal Concentration (MFC) for Candida albicans (ATCC 90029), Candida albicans (CBS 562), Candida tropicalis (ATCC 705) and Candida tropicalis strains (CBS 94), as well as to analyze the possible mechanism of action of the oil through the addition of sorbitol to the culture medium. Methods. For the MIC determination, inocula were previously adjusted through spectrophotometry and 100μL were added to the wells of plates already containing the culture medium and 100μL of the serial dilutions of the oil, incubating them in aerobiosis for 24 hours, with subsequent staining by 1% TCT. For the MFC, 50μL of the supernatant from the MIC assay wells were dripped onto Petri dishes and incubated in aerobiosis for 24 hours. Tests were performed in triplicate and data analysed by descriptive statistics. Results. It was determined that the MIC for C. albicans was 125 μg/mL while MIC for C. tropicalis was 250 μg/mL, with the essential oil presenting fungicidal effect for both analyzed yeasts. Conclusion. The essential oil of Cymbopogon citratus does not act at the cellular wall level and demonstrated an antimicrobial effect on Candida albicans and Candida tropicalis, therefore acting as a fungicide.

Author Biography

Naiana Braga da Silva, State University of Paraiba, Campina Grande.

Editor de Journal of Oral Research.

Asesor en Metodología de la Investigación.

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Published
2017-12-22
How to Cite
DA SILVA, Naiana Braga et al. Antifungal Activity of the Essential Oil of Cymbopogon citratus (DC) Stapf. An in vitro study.. Journal of Oral Research, [S.l.], v. 6, n. 12, p. 319-323, dec. 2017. ISSN 0719-2479. Available at: <https://joralres.com/index.php/JOralRes/article/view/joralres.2017.092>. Date accessed: 29 mar. 2024. doi: https://doi.org/10.17126/joralres.2017.092.
Section
Articles

Keywords

phytotherapy; microbial sensitivity test; candidiasis.