TY - JOUR AU - Huerta, Elisa AU - Gorup, Daisy AU - Manríquez, Manuel AU - Choappa, Rodrigo Cruz AU - Vieille, Peggy AU - González-Arriagada, Wilfredo PY - 2020/02/29 TI - Efficacy of copper sulphate on Candida albicans on heat-polymerized acrylic resin. JF - Journal of Oral Research; Vol 9 No 1 (2020)DO - 10.17126/joralres.2020.009 KW - N2 - The ageing of population is increasing, and a great percentage of these patients wear removable prostheses, and can suffer denture stomatitis, a condition that has been associated with candidiasis. Aims: To evaluate in vitro the effectiveness of Copper Sulfate against Candida albicans in samples of heat-polymerized acrylic resin, compared to nystatin, sodium hypochlorite and chlorhexidine.  Materials and Methods: Initially, the minimum inhibitory concentration (MIC) of copper sulfate for Candida albicans was determined by microdilution. Then, 54 resin samples were divided into 6 treatment groups corresponding to Nystatin 100.000 UI, Sodium Hypochlorite 0.5%, chlorhexidine 0.12%, Copper Sulfate 4.7µg/ml, Copper Sulfate 9.4µg/ml and physiological saline solution, in which samples were submerged for 6 hours. Resin samples were then washed and cultured on solid media at 37°C for 72 hours. The number of colony-forming units was determined using a Quebec colony counter. The statistical analysis was performed using the Kruskal-Wallis test and the Mann-Whitney U test.  Results: Copper sulfate at a concentration of 9.4μg/ml presented a similar effectiveness as the other control products regarding the reduction in the number of colonies of Candida albicans post-treatment. Conclusion: The effectiveness of copper sulfate against Candida albicans on acrylic resin was similar to that of nystatin, sodium hypochlorite and chlorhexidine. UR - https://joralres.com/index.php/JOralRes/article/view/joralres.2020.009