Biological and physico-mechanical properties of poly(methyl methacrylate) enriched with graphene oxide as a potential biomaterial.

  • René García-Contreras Escuela Nacional de Estudios Superiores (ENES) Unidad León, Universidad Nacional Autónoma de México (UNAM), León, Gto., México; Osforma, Los Algodones, B.C., México.
  • Héctor Guzmán-Juárez Osforma, Los Algodones, B.C., México
  • Daniel López-Ramos TPD Práctica privada.
  • Carlos Alvarez-Gayosso Osforma, Los Algodones, B.C., México; DEPEI-UNAM, Laboratorio Materiales Dentales, Cd. México, México.

Abstract

Objective: To determine the cytotoxicity and effects of graphene oxide (GO) on cellular proliferation of gingival-fibroblasts, pulpdental cells and human osteoblasts in culture, and to determine the physical, mechanical and biological properties of poly (methyl methacrylate) (PMMA) enriched with GO. Material and Methods: T he G O w as c haracterized with SEM. Cytotoxicity and cell proliferation were determined by the MTT bioassay. The physical-mechanical tests (flexural strength and elastic  modulus) were carried out with a universal testing machine. Sorption and solubility were determined by weighing before and after drying and immersion in water. Porosity was evaluated by visual inspection. Data were analyzed with Student's t-test and Tukey's post-hoc ANOVA. Results: The GO has a heterogeneous morphology and a particle size of 66.67±64.76 μm. GO has a slight to no-cytotoxicity (>50-75% viability) at 1-30 days, and at 24 hours incubation of PMMA with GO significantly stimulates osteoblasts (45±8%, p<0.01). The physical and mechanical properties of PMMA with GO increase considerably without altering sorption, solubility and porosity. Conclusion: GO alone or with PMMA has an acceptable biocompatibility, could contribute to cell proliferation, cell regeneration and improve the physical-mechanical properties of PMMA.
Published
2021-04-30
How to Cite
GARCÍA-CONTRERAS, René et al. Biological and physico-mechanical properties of poly(methyl methacrylate) enriched with graphene oxide as a potential biomaterial.. Journal of Oral Research, [S.l.], v. 10, n. 2, p. 1-9, apr. 2021. ISSN 0719-2479. Available at: <https://joralres.com/index.php/JOR/article/view/joralres.2021.019>. Date accessed: 24 july 2021. doi: https://doi.org/10.17126/joralres.2021.019.
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Articles