Special care for oral tissue samples after biopsy: Proper storage and transport — A comparative Study

  • Amjad Azizzadeh Faculty of Dentistry, Kurdistan University of Medical Sciences, Sanandaj, Iran
  • Massoumeh Zargaran Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Kurdistan University of Medical Sciences, Sanandaj, Iran
  • Varisheh Rastin Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Kurdistan University of Medical Sciences, Sanandaj, Iran
  • Bijan Nouri Social Determinants of Health Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran


Objective: Biopsy is the gold standard for the diagnosis of oral lesions. Correct management and care of biopsy at all steps (before, during and after obtaining a biopsy) is highly important to provide proper tissue samples for microscopic assessment by pathologists. This study aimed to assess and compare the knowledge of dental students (DSs) and general dentists (GDs) on post-oral biopsy instructions. Material and Methods: A questionnaire including two parts was used: 1) Demographic data and self-evaluation of biopsy knowledge by the participants and 2) 11 items about the correct oral biopsy storage and transport to a histopathology laboratory. The data collected from the questionnaires were analyzed by STATA. Results: 48 GDs and 131 DSs participated in this study. The knowledge score of the DSs (5.43±2.01) was significantly lower than GDs (8.33±1.78) (p<0.05). Moreover, there was no significant relationship between GDs' knowledge and their working experience, age, gender and the university they graduated from. However, there was a significant relationship between DSs' school year and their knowledge. Conclusion: The findings showed that the knowledge of DSs was lower than GDs. Since, these students will care for the oral and dental health of the community in the future, upgrading their training (by improving the quantity and quality of theoretical and practical training) is necessary to both understand the different aspects of biopsy, and to be familiar enough with proper oral biopsy storage and transport processes.


1. Kumaraswamy KL, Vidhya M, Rao PK, Mukunda A. Oral biopsy: oral pathologist's perspective. J Cancer Res Ther. 2012;8(2):192-8.
2. Poh CF, Ng S, Berean KW, Williams PM, Rosin MP, Zhang L. Biopsy and histopathologic diagnosis of oral premalignant and malignant lesions. J Can Dent Assoc. 2008;74(3):283-8.
3. Rastogi V, Puri N, Arora S, Kaur G, Yadav L, Sharma R. Artefacts: a diagnostic dilemma - a review. J Clin Diagn Res. 2013;7(10):2408-13.
4. Jain N. Essentials before Sending Biopsy Specimens: A Surgeon's Prespective and Pathologists Concern. J Maxillofac Oral Surg. 2011;10(4):361-4.
5. Logan RM, Goss AN. Biopsy of the oral mucosa and use of histopathology services. Aust Dent J. 2010;55:9-13.
6. Wan A, Savage NW. Biopsy and diagnostic histo-pathology in dental practice in Brisbane: usage patterns and perceptions of usefulness. Aust Dent J. 2010;55(2):162-169.
7. Camacho Alonso F, López Jornet P, Jiménez Torres MJ, Orduña Domingo A. Analysis of the histopathological artefacts in punch biopsies of the normal oral mucosa. Med Oral Patol Oral Cir Bucal. 2008;13(10):E636-9.
8. Uti OG, Fashina AA. Oral cancer education in dental schools: knowledge and experience of Nigerian undergraduate students. J Dent Educ. 2006;70(6): 676-80.
9. López Jornet P, Velandrino Nicolás A, Martínez Beneyto Y, Fernández Soria M. Attitude towards oral biopsy among general dentists in Murcia. Med Oral Patol Oral Cir Bucal. 2007; 12(2): 116-21.
10. Murgod V, Angadi P, Hallikerimath S, Kale A, Hebbal M. Attitudes of general dental practitioners towards biopsy procedures. J Clin Exp Dent. 2011;3(5):e418-23.
11.Bataineh AB, Hammad HM, Darweesh IA. Attitude toward oral biopsy among general dental practitioners: Awareness and practice. J Orofac Sci. 2015; 7(1):19-26.
12. Hunasgi S, Koneru A, Manvikar V, Amrutha R, Reddy KMP. Oral biopsy in general dental practiceSantosh. JCRI. 2017; 4(5), 162–5.
13. Dimenstein IB. Grossing biopsies: an introduction to general principles and techniques. Ann Diagn Pathol. 2009;13(2):106-13.
14. Oliver RJ, Sloan P, Pemberton MN. Oral biopsies: methods and applications. Br Dent J. 2004;196(6):329-333.
15. Bindhu P, Krishnapillai R, Thomas P, Jayanthi P. Facts in artifacts. J Oral Maxillofac Pathol . 2013 ;17(3):397-401.
16. Chatterjee S. Artefacts in histopathology. J Oral Maxillofac Pathol. 2014;18(Suppl 1)::S111-6.
17. Sengupta S, Prabhat K, Gupta V, Vij H, Vij R, Sharma V. Artefacts produced by normal saline when used as a holding solution for biopsy tissues in transit. J Maxillofac Oral Surg. 2014;13(2):148-51.
18. sharif MA, Mushtaq S, Mamoon N, Jamal S, Luqman M. Clinician’s Responsibility in pre-analytical quality assurance of histopathology. Pak J Med Sci. 2007;23:720-3
19. Comanescu M, Annaratone L, D'Armento G, Cardos G, Sapino A, Bussolati G. Critical steps in tissue processing in histopathology. Recent Pat DNA Gene Seq. 2012;6(1):22-32.
20. Comănescu M, Arsene D, Ardeleanu C, Bussolati G. The mandate for a proper preservation in histopathological tissues. Rom J Morphol Embryol. 2012;53(2):233-242.
21. Wray S. Containers for specimens. Br Med J. 1969;4(5675):113
22. Jephcott A. The surgical management of the oral soft tissues: 3. Biopsy. Dent Update. 2007; 34(10): 654-57.
23. Melrose RJ, Handlers JP, Kerpel S, Summerlin DJ, Tomich CJ. American Academy of Oral and Maxillofacial Pathology. The use of biopsy in dental practice. The position of the American Academy of Oral and Maxillofacial Pathology. Gen Dent. 2007;55(5):457-61.
How to Cite
AZIZZADEH, Amjad et al. Special care for oral tissue samples after biopsy: Proper storage and transport — A comparative Study. Journal of Oral Research, [S.l.], v. 10, n. 1, p. 1-8, feb. 2021. ISSN 0719-2479. Available at: <https://joralres.com/index.php/JOR/article/view/joralres.2021.012>. Date accessed: 24 july 2021. doi: https://doi.org/10.17126/joralres.2021.012.