Dental stem cells and their application in dentistry.

  • Heber Arbildo-Vega Facultad de Odontología, Universidad San Martín de Porres. Chiclayo, Perú. /Escuela de Odontología, Universidad Particular de Chiclayo. Chiclayo, Perú./ Centro de Salud Odontológico San Mateo. Trujillo, Perú.
  • Fredy Cruzado-Oliva Facultad de Estomatología, Universidad Nacional de Trujillo. Trujillo, Perú.
  • Edward Infantes-Ruiz Escuela de Odontología, Universidad Particular de Chiclayo. Chiclayo, Perú.

Abstract

Recent advances in tissue engineering and regenerative medicine offer a long-term solution through biological repair, replacement of damaged teeth or maintenance and improvement of tissue and organ function through the use of stem cells. Stem cells or also called universal cells, progenitor cells or precursor cells; they are primitive, undifferentiated, clonogenic cells that are characterized by their self-renewal capabilities and that can be differentiated into more specialized cells with specific functions. Currently many sources are known from where you can obtain stem cells, one of which are those obtained from oral or dental tissues, called dental stem cells (DSC), from where it has been possible to identify, isolate and characterize around 8 unique populations: dental pulp stem cells (DPSC), human exfoliated deciduous tooth stem cells (SHED), periodontal ligament stem cells (PLDSC), dental follicle stem cells (DFSC), stem cells derived from bone alveolar (CMHA), the stem cells of the apical papilla (SCAP), the stem cells of the dental germ (DGSC) and the gingival stem cells (GSC). These DSC have attracted attention in recent years due to their accessibility, plasticity and high proliferation capacity. Currently, DSC have shown that they can be used in endodontic and periodontal regenerative therapy, in the regeneration of dentin and bone and in dental bioengineering. Tissue engineering methodologies combined with a greater understanding of the biology of DSCs will provide powerful tools for a broader spectrum of their application in various future therapeutic strategies.

References

1. Chalisserry EP, Nam SY, Park SH, Anil S. Therapeutic potential of dental stem cells. J Tissue Eng. 2017; 8: 2041731417702531.
2. Sharpe PT. Dental mensenchymal stem cells. Development. 2016; 143(13): 2273-80.
3. Mitsiadis TA, Orsini G, Jimenez-Rojo L. Stem cell-based approaches in dentistry. Eur Cell Mater. 2015; 30: 248-57.
4. Zhai Q, Dong Z, Wang W, Li B, Jin Y. Dental stem cell and dental tissue regeneration. Front Med. 2019; 13(2): 152-9.
5. Hu L, Liu Y, Wang S. Stem cell-based tooth and periodontal regeneration. Oral Dis. 2018; 24(5): 696-705.
6. Miran S, Mitsiadis TA, Pagella P. Innovate dental stem cell-based research approaches: The future of Dentistry. Stem Cells Int. 2016; 2016: 7231038.
7. Mao X, Liu Y, Chen C, Shi S. Mesenchymal stem cells and their role in dental medicine. Dent Clin North Am. 2017; 61(1): 161-72.
8. Asutay F, Acar HA, Yolcu U, Kirtay M, Alan H. Dental stem cell sources and their potentials for bone tissue engineering. J Istanb Univ Fac Dent. 2015; 49(2): 51-6.
9. Morsczeck C, Reichert TE. Dental stem cells in tooth regeneration and repair in the future. Expert Opin Biol Ther. 2018; 18(2): 187-96.
10. Cui D, Li H, Wan M, Peng Y, Xu X, Zhou X, Zheng L. The origin and identification of mesenchymal stem cells in teeth: from odontogenic to non-odontogenic. Curr Stem Cell Res Ther. 2018; 13(1): 39-45.
11. Zhang W, Ouyang H, Dass CR, Xu J. Current research on pharmacologic and regenerative therapies for osteoarthritis. Bone Ress. 2016; 4: 15040.
12. Chen YY, He ST, Yan FH, Zhou PF, Luo K, Zhang YD, Xiao Y, Lin MK. Dental pulp stem cells express tendon markers under mechanical loading and are a potential cell source for tissue engineering of tendon-like tissue. Int J Oral Sci. 2016; 8(4): 213-22.
13. Pilipchuk SP, Plonka AB, Monje A, Taut AD, Lanis A, Kang B, Giannobile WV. Tissue engineering for bone regeneration and osseointegration in the oral cavity. Dent Mater. 2015; 31(4): 317-38.
14. Mao AS, Mooney DJ. Regenerative medicine: current therapies and future directions. Proc Natl Acad Sci U S A. 2015; 112(47): 14452-9.
15. Bansal R, Jain A. Current overview on dental stem cells applications in regenative dentistry. J Nat Sci Biol Med. 2015; 6(1): 29-34.
16. Abdel Meguid E, Ke Y, Ji J, El-Hashash AHK. Stem cells applications in bone and tooth repair and regeneration: New insights, tools, and hopes. J Cell Physiol. 2018; 233(3): 1825-35.
17. Har A, Park JC. Dental Stem cells and their applications. Chin J Dent Res. 2015; 18(4): 207-12.
18. Baniebrahimi G, Khanmohammadi R, Mir F. Teeth-derived stem cells: A source for cell therapy. J Cell Physiol. 2019; 234(3): 2426-35.
19. Khazaei M, Bozorgi A, Khazaei S, Khademi A. Stem cells in dentistry, sources and applications. Dent Hypotheses. 2016; 7: 42-52.
20. Goradel NH, Hour FG, Negahdari B, Malekshahi ZV, Hashemzehi M, Masoudifar A, Mirzaei H. Stem cell therapy: A new therapeutic option for cardiovascular diseases. J Cell Biochem. 2018; 119(1): 95-104.
21. Mirzaei H, Sahebkar A, Avan A, Jaafari MR, Salehi R, Salehi H, Baharvand H, Rezaei A, Hadjati J, Pawelek JM, Mirzaei HR. Application of mesenchymal stem cells in melanoma: A potential therapeutic strategy for delivery of targeted agents. Curr Med Chem. 2016; 23(5): 455-63.
22. Mohammadi M, Jaafari MR, Mirzaei HR, Mirzaei H. Mesenchymal stem cell: A new horizon in cancer gene therapy. Cancer Gene Ther. 2016; 23(9): 285-6.
23. Mirzaei H, Sahebkar A, Sichani LS, Moridikia A, Nazari S, Sadri Nahand J, Salehi H, Stenvang J, Masoudifar A, Mirzaei HR, Jaafari MR. Therapeutic application of multipotent stem cells. J Cell Physiol. 2018; 233(4): 2815-23.
24. Mirzaei H, Salehi H, Oskuee RK, Mohammadpour A, Mirzaei HR, Sharifi MR, Salarinia R, Darani HY, Mokhtari M, Masoudifar A, Sahebkar A, Salehi R, Jaafari MR. The therapeutic potential of human adipose-derived mesenchymal stem cells producing CXCL 10 in a mouse melanoma lung metastasis model. Cancer Lett. 2018; 419: 30-9.
25. Moradian Tehrani R, Verdi J, Noureddini M, Salehi R, Salarinia R, Mosalaei M, Simonian M, Alani B, Ghiasi MR, Jaafari MR, Mirzaei HR, Mirzaei H. Mesenchymal stem cells: A new platform for targeting suicide genes in cancer. J Cell Physiol. 2018; 233(5): 3831-45.
26. Moradian Tehrani R, Mirzaei H, Verdi J, Sahebkar A, Noureddini M, Salehi R, Alani B, Kianmehr M. Chondrogenic differentiation of human scalp adipose-derived stem cells in Polycaprolactone scaffold and using freeze thaw freeze method. J Cell Physiol. 2018; 233(10): 6705-13.
27. Liu J, Yu F, Sun Y, Jiang B, Zhang W, Yang J, Xu GT, Liang A, Liu S. Concise reviews: Characteristics and potential applications of human dental tissue-derived mesenchymal stem cells. Stem Cells. 2015; 33(3): 627-38.
28. Nuti N, Corallo C, Chan BM, Ferrari M, Gerami-Naini B. Multipotent differentiation of human dental pulp stem cells: a literature review. Stem Cell Rev. 2016; 12(5): 511-23.
29. Lee DJ, Tseng HC, Wong SW, Wang Z, Deng M, Ko CC. Dopaminergic effects on in vitro osteogenesis. Bone Res. 2015; 3: 15020.
30. Maharam E, Yaport M, Villanueva NL, Akinyibi T, Laudier D, He Z, Leong DJ, Sun HB. Rho/Rock signal transduction pathway is required for MSC tenogenic differentiation. Bone Res. 2015; 3: 15015.
31. Sedqley CM, Botero TM. Dental stem cells and their sources. Dent Clin North Am. 2012; 56(3): 549-61.
32. La Noce M, Mele L, Tirino V, Paino F, De Rosa A, Naddeo P, Papagerakis P, Papaccio G, Desiderio V. Neural crest stem cell population in craniomaxillofacial development and tissue repair. Eur Cell Mater. 2014; 28: 348–57.
33. Matsubara K, Matsushita Y, Sakai K, Kano F, Kondo M, Noda M, Hashimoto N, Imagama S, Ishiguro N, Suzumura A, Ueda M, Furukawa K, Yamamoto A. Secreted ectodomain of sialic acid-binding Ig-like lectin-9 and monocyte chemoattractant protein-1 promote recovery after rat spinal cord injury by altering macrophage polarity. J Neurosci. 2015; 35(6): 2452–64.
34. Pisciotta A, Carnevale G, Meloni S, Riccio M, De Biasi S, Gibellini L, Ferrari A, Bruzzesi G, De Pol A. Dental pulp-derived stem cell conditioned medium reduces cardiac injury following ischemia-reperfusion. Sci Rep. 2015; 5: 16295.
35. Pisciotta A, Carnevale G, Meloni S, Riccio M, De Biasi S, Gibellini L, Ferrari A, Bruzzesi G, De Pol A. Human dental pulp stem cells (hDPSCs): isolation, enrichment and comparative differentiation of two sub-populations. BMC Dev Biol. 2015; 15:14.
36. Patil R, Kumar BM, Lee WJ, Jeon RH, Jang SJ, Lee YM, Park BW, Byun JH, Ahn CS, Kim JW, Rho GJ. Multilineage potential and proteomic profiling of human dental stem cells derived from a single donor. Exp Cell Res. 2014; 320: 92-107.
37. Alvarez R, Lee HL, Hong C, Wang CY. Single CD271 marker isolates mesenchymal stem cells from human dental pulp. Int J Oral Sci. 2015; 7(4): 205-12.
38. Alvarez R, Lee HL, Wang CY, Hong C. Characterization of the osteogenic potential of mesenchymal stem cells from human periodontal ligament based on cell surface markers. Int J Oral Sci. 2015; 7(4): 2013-9.
39. Rombouts C, Giraud T, Jeanneau C, About I. Pulp vascularization during tooth development, regeneration, and therapy. J Dent Res. 2017; 96(2): 137-44.
40. Yasui T, Mabuchi Y, Toriumi H, Ebine T, Niibe K, Houlihan DD, Morikawa S, Onizawa K, Kawana H, Akazawa C, Suzuki N, Nakagawa T, Okano H, Matsuzaki Y. Purified human dental pulp stem cells promote osteogenic regeneration. J Dent Res. 2016; 95(2): 206-14.
41. Paino F, Ricci G, De Rosa A, D'Aquino R, Laino L, Pirozzi G, Tirino V, Papaccio G. Ecto-mesenchymal stem cells from dental pulp are committed to differentiate into active melanocytes. Eur Cell Mater. 2010; 20: 295–305.
42. Davies OG, Cooper PR, Shelton RM, Smith AJ, Scheven BA. A comparison of the in vitro mineralisation and dentinogenic potential of mesenchymal stem cells derived from adipose tissue, bone marrow and dental pulp. J Bone Miner Metab. 2015; 33(4): 371–82.
43. Martinez SD, Sasaki RT, Neves AD, da Silva MC. Stem cells from human exfoliated deciduous teeth: a growing literature. Cells Tissues Organs. 2016; 202(5-6): 269-80.
44. Miura M, Gronthos S, Zhao M, Lu B, Fisher LW, Robey PG, Shi S. SHED: stem cells from human exfoliated deciduous teeth. Proc Natl Acad Sci USA. 2003; 100(10): 5807–12.
45. Liu Y, Chen C, Liu S, Liu D, Xu X, Chen X, Shi S. Acetylsalicylic acid treatment improves differentiation and immunomodulation of SHED. J Dent Res. 2015; 94(1): 209-18.
46. Liu Y, Wang L, Liu S, Liu D, Chen C, Xu X, Chen X, Shi S. Transplantation of SHED prevents bone loss in the early phase of ovariectomy-induced osteoporosis. J Dent Res. 2014; 93(11): 1124-32.
47. Liu D, Xu J, Liu O, Fan Z, Liu Y, Wang F, Ding G, Wei F, Zhang C, Wang S. Mesenchymal stem cells derived from inflamed periodontal ligaments exhibit impaired immunomodulation. J Clin Periodontol. 2012; 39(12): 1174-82.
48. Shoi K, Aoki K, Ohya K, Takagi Y, Shimokawa H. Characterization of pulp and follicle stem cells from impacted supernumerary maxillary incisors. Pediatr Dent. 2014; 36(3): 79-84.
49. Matsubara T, Suardita K, Ishii M, Sugiyama M, Igarashi A, Oda R, Nishimura M, Saito M, Nakagawa K, Yamanaka K, Miyazaki K, Shimizu M, Bhawal UK, Tsuji K, Nakamura K, Kato Y. Alveolar bone marrow as a cell source for regenerative medicine: differences between alveolar and iliac bone marrow stromal cells. J Bone Miner Res. 2005; 20(3): 399–409.
50. Mason S, Tarle SA, Osibin W, Kinfu Y, Kaigler D. Standardization and safety of alveolar bone-derived stem cell isolation. J Dent Res. 2014; 93(1): 55–61.
51. Lim KT, Hexiu J, Kim J, Seonwoo H, Choung PH, Chung JH. Synergistic effects of orbital shear stress on in vitro growth and osteogenic differentiation of human alveolar bone-derived mesenchymal stem cells. Biomed Res Int. 2014; 2014: 316803.
52. Du L, Yang P, Ge S. Isolation and characterization of human gingiva-derived mesenchymal stem cells using limiting dilution method. J Den Sci. 2016; 11(3): 304–14.
53. Gong T, Xie J, Liao J, Zhang T, Lin S, Lin Y. Nanomaterials and bone regeneration. Bones Res. 2015; 3: 15029.
54. Yi H, Rehman FU, Zhao C, Liu B, He N. Recent advances in nano scaffolds for bone repair. Bone Res. 2016; 4: 16050.
55. Liu M, Zeng X, Ma C, Yi H, Ali Z, Mou X, Li S, Deng Y, He N. Injectable hydrogels for cartilage and bone tissue engineering. Bone Res. 2017; 5: 17014.
56. Yu X, Ge S, Chen S, Xu Q, Zhang J, Guo H, Yang P. Human gingiva-derived mesenchymal stromal cells contribute to periodontal regeneration in beagle dogs. Cells Tissues Organs. 2013; 198(6): 428-37.
57. Li Y, Zhao S, Nan X, Wei H, Shi J, Li A, Gou J. Repair of human periodontal bone defects by autologous grafting stem cells derived from inflammatory dental pulp tissues. Stem Cells Res Ther. 2016; 7: 141.
58. Zhu B, Liu W, Liu Y, Zhao X, Zhang H, Luo Z, Jin Y. Jawbone microenvironment promotes periodontium regeneration by regulating the function of periodontal ligament stem cells. Sci Rep. 2017; 7: 40088.
59. Nagata M, Iwasaki K, Akazawa K, Komaki M, Yokoyama N, Izumi Y, Morita I. Conditioned medium from periodontal ligament stem cells enhances periodontal regeneration. Tissue Eng Part A. 2017; 23(9-10): 367-77.
60. Lucaciu O, Soriţău O, Gheban D, Ciuca DR, Virtic O, Vulpoi A, Dirzu N, Câmpian R, Băciuţ G, Popa C, Simon S, Berce P, Băciuţ M, Crisan B. Dental follicle stem cells in bone regeneration on titanium implants. BMC Biotechnol. 2015; 15: 114.
61. Zhang Q, Nguyen AL, Shi S, Hill C, Wilder-Smith P, Krasieva TB, Le AD. Three-dimensional spheroid culture of human gingiva-derived mesenchymal stem cells enhances mitigation of chemotherapy-induced oral mucositis. Stem Cells Dev. 2012; 21(6): 937-47.
62. Gao Y, Zhao G, Li D, Chen X, Pang J, Ke J. Isolation and multiple differentiation potential assessment of human gingival mesenchymal stem cells. Int J Mol Sci. 2014; 15(11): 20982-96.
63. Fawzy El-Sayed KM, Mekhemar MK, Beck-Broichsitter BE, Bähr T, Hegab M, Receveur J, et al. Periodontal regeneration employing gingival margin-derived stem/progenitor cells in conjunction with IL-1ra-hydrogel synthetic extracellular matrix. J Clin Periodontol. 2015; 42(5): 448-57.
64. Xuan K, Li B, Guo H, Sun W, Kou X, He X, et al. Deciduous autologous tooth stem cells regenerate dental pulp after implantation into injured teeth. Sci Transl Med. 2018; 10(455): eaaf3227.
65. Ferrarotti F, Romano F, Gamba MN, Quirico A, Giraudi M, Audagna M, et al. Human intrabony defect regeneration with micrografts containing dental pulp stem cells: A randomized controlled clinical trial. J Clin Periodontol. 2018; 45(7): 841‐50.
66. Chen FM, Gao LN, Tian BM, Zhang XY, Zhang YJ, Dong GY, Lu H, Chu Q, Xu J, Yu Y, Wu RX, Yin Y, Shi S, Jin Y. Treatment of periodontal intrabony defects using autologous periodontal ligament stem cells: a randomized clinical trial. Stem Cell Res Ther. 2016;7:33.
67. d'Aquino R, De Rosa A, Lanza V, Tirino V, Laino L, Graziano A, Desiderio V, Laino G, Papaccio G. Human mandible bone defect repair by the grafting of dental pulp stem/progenitor cells and collagen sponge biocomplexes. Eur Cell Mater. 2009;12;18:75-83.
68. Giuliani A, Manescu A, Langer M, Rustichelli F, Desiderio V, Paino F, De Rosa A, Laino L, d'Aquino R, Tirino V, Papaccio G. Three years after transplants in human mandibles, histological and in-line holotomography revealed that stem cells regenerated a compact rather than a spongy bone: biological and clinical implications. Stem Cells Transl Med. 2013; 2(4): 316-24.
69. Gault P, Black A, Romette JL, Fuente F, Schroeder K, Thillou F, Brune T, Berdal A, Wurtz T. Tissue-engineered ligament: implant constructs for tooth replacement. J Clin Periodontol. 2010; 37(8): 750-8.
70. Feng F, Akiyama K, Liu Y, Yamaza T, Wang TM, Chen JH, Wang BB, Huang GT, Wang S, Shi S. Utility of PDL progenitors for in vivo tissue regeneration: a report of 3 cases. Oral Dis. 2010; 16(1): 20-8.
71. Gao ZH, Hu L, Liu GL, Wei FL, Liu Y, Liu ZH, Fan ZP, Zhang CM, Wang JS, Wang SL. Bio-Root and Implant-Based Restoration as a Tooth Replacement Alternative. J Dent Res. 2016; 95(6): 642–9.
72. Hung CN, Mar K, Chang HC, Chiang YL, Hu HY, Lai CC, Chu RM, Ma CM. A comparison between adipose tissue and dental pulp as sources of MSCs for tooth regeneration. Biomaterials. 2011; 32(29): 6995–7005.
73. Zhang W, Vazquez B, Yelick PC. Bioengineered post-natal recombinant tooth bud models. J Tissue Eng Regen Med. 2017; 11(3): 658–68.
74. Yang B, Chen G, Li J, Zou Q, Xie D, Chen Y, Wang H, Zheng X, Long J, Tang W, Guo W, Tian W. Tooth root regeneration using dental follicle cell sheets in combination with a dentin matrix-based scaffold. Biomaterials. 2012; 33(8): 2449-61.
75. Nakashima M, Iohara K. Mobilized dental pulp stem cells for pulp regeneration: initiation of clinical trial. J Endod. 2014; 40(4. Suppl): S26–32
76. Nakashima M, Iohara K, Murakami M, Nakamura H, Sato Y, Ariji Y, Matsushita K.Pulp regeneration by transplantation of dental pulp stem cells in pulpitis: a pilot clinical study. Stem Cell Res Ther. 2017; 8(1): 61.
77. Rosa V, Zhang Z, Grande RH, Nör JE. Dental pulp tissue engineering in full-length human root canals. J Dent Res. 2013; 92(11): 970–5.
78. Potdar PD, Jethmalani YD. Human dental pulp stem cells: applications in future regenerative medicine. World J Stem Cells 2015; 7(5): 839–51.
79. Wang X, He H, Wu X, Hu J, Tan Y. Promotion of dentin regeneration via CCN3 modulation on Notch and BMP signaling pathways. Biomaterials 2014; 35(9): 2720–9.
80. Albuquerque MT, Valera MC, Nakashima M, Nör JE, Bottino MC. Tissue-engineering-based strategies for regenerative endodontics. J Dent Res. 2014; 93(12): 1222–31.
81. Wang W, Dang M, Zhang Z, Hu J, Eyster TW, Ni L, Ma PX. Dentin regeneration by stem cells of apical papilla on injectable nanofibrous microspheres and stimulated by controlled BMP-2 release. Acta Biomater. 2016; 36: 63–72.
82. Chiang YC, Chang HH, Wong CC, Wang YP, Wang YL, Huang WH, Lin CP. Nanocrystalline calcium sulfate/hydroxyapatite biphasic compound as a TGF-beta1/VEGF reservoir for vital pulp therapy. Dent Mater. 2016; 32(10): 1197–1208.
83. Yang B, Qiu Y, Zhou N, Ouyang H, Ding J, Cheng B, Sun J. Effects of human vascular endothelial growth factor on reparative dentin formation. Mol Med Rep. 2016; 13(1): 705–12.
84. Song JS, Takimoto K, Jeon M, Vadakekalam J, Ruparel NB, Diogenes A. Decellularized human dental pulp as a scaffold for regenerative endodontics. J Dent Res. 2017; 96(6): 640-6
85. Huojia M, Wu Z, Zhang X, Maimaitiyiming M, Rong M. Effect of dental pulp stem cells (DPSCs) in repairing rabbit alveolar bone defect. Clin Lab. 2015; 61(11): 1703-8.
86. Yang B, Qiu Y, Zhou N, Ouyang H, Ding J, Cheng B, Sun J. Application of Stem Cells in Oral Disease Therapy: Progresses and Perspectives. Front Physiol. 2017; 8:197.
87. Honda MJ, Imaizumi M, Tsuchiya S, Morsczeck C. Dental follicle stem cells stem cells and tissue engineering. J Oral Sci. 2010; 52(4): 541-52.
88. Szepesi A, Matula Z, Szigeti A, Várady G, Szalma J, Szabó G, Uher F, Sarkadi B, Német K. In vitro characterization of human mesenchymal stem cells isolated from different tissues with a potential to promote complex bone regeneration. Stem Cells Int. 2016; 3595941.
89. Flores MG, Hasegawa M, Yamato M, Takagi R, Okano T, Ishikawa I. Cementum-periodontal ligament complex regeneration using the cell sheet technique. J Periodontal Res. 2008; 43(3): 364–71.
90. Chen FM, Gao LN, Tian BM, Zhang XY, Zhang YJ, Dong GY, Lu H, Chu Q, Xu J, Yu Y, Wu RX, Yin Y, Shi S, Jin Y. Treatment of periodontal intrabony defects using autologo us periodontal ligament stem cells: a randomized clinical trial. Stem Cell Res Ther. 2016; 7(1): 33.
91. Tassi SA, Sergio NZ, Misawa MYO, Villar CC. Efficacy of stem cells on periodontal regeneration: systematic review of pre-clinical studies. J Periodontol Res. 2017; 52(5): 793-812.
92. Baik HS, Park J, Lee KJ, Chung C. Local application of periodontal ligament stromal cells promotes soft tissue regeneration. Oral Dis. 2014; 20(6): 574-81.
93. Du J, Shan Z, Ma P, Wang S, Fan Z. Allogeneic bone marrow mesenchymal stem cell transplantation for periodontal regeneration. J Dent Res. 2014; 93(2): 183-8.
94. Owaki T, Shimizu T, Yamato M, Okano T. Cell sheet engineering for regenerative medicine: current challenges and strategies. Biotechnol J. 2014; 9(7): 904-14.
95. Hu J, Cao Y, Xie Y, Wang H, Fan Z, Wang J, Zhang C, Wang J, Wu CT, Wang S. Periodontal regeneration in swine after cell injection and cell sheet transplantation of human dental pulp stem cells following good manufacturing practice. Stem Cell Res Ther. 2016; 7(1): 130.
96. Fu X, Jin L, Ma P, Fan Z, Wang S. Allogeneic stem cells form deciduous teeth in treatment for periodontitis in miniature swine. J Periodontol. 2014; 85(6): 845-51.
Published
2020-06-30
How to Cite
ARBILDO-VEGA, Heber; CRUZADO-OLIVA, Fredy; INFANTES-RUIZ, Edward. Dental stem cells and their application in dentistry.. Journal of Oral Research, [S.l.], v. 9, n. 3, p. 220-233, june 2020. ISSN 0719-2479. Available at: <http://joralres.com/index.php/JOR/article/view/joralres.2020.039>. Date accessed: 27 oct. 2020. doi: https://doi.org/10.17126/joralres.2020.039.
Section
Reviews