Evaluation of serum and salivary transforming growth factor beta, vascular endothelial growth factor and tumor necrosis factor alpha in oral lichen planus.

  • Amira Abdelwhab Abdelghaffar Cairo University, Cairo, Egypt.| October 6 University, Giza, Egypt.
  • Mahmoud Mohamed Kandeel Cairo University, Cairo, Egypt.
  • Hala Hassan Yassin October 6 University, Giza, Egypt
  • Wesam Abdelmonem Cairo University, Cairo, Egypt
  • Olfat Gamil Shaker Cairo University, Cairo, Egypt

Abstract

Introduction: Lichen planus is one of the most common oral mucosal lesions. Transforming growth factor-β (TGF- β) has a marked effect on epithelial–mesenchymal transition and immune cells function. Vascular Endothelial Growth Factor (VEGF) is a key regulator of vasculogenesis and angiogenesis. Tumor necrosis factor-α (TNF-α) mediates T-lymphocyte homing and apoptosis of epithelial cells. Objetive: The present study was conducted in order to compare the expression of serum and salivary TGF- β, VEGF, TNF-α between OLP patients and control individuals to investigate if saliva can be used as an alternative to serum for diagnostic purposes and for monitoring disease. Materials and Methods: 23 OLP patients and 23 control individuals were included to evaluate serum and salivary TGF-β, VEGF, TNF-α using ELISA kits. Five milliliters of venous blood was collected and unstimulated saliva was collected by the spitting method. Results: Serum and salivary levels of TGF- β, VEGF, TNF-α are higher in OLP patients compared to normal controls. Mean difference is higher in saliva than serum. Moreover, there was a significant difference in serum and salivary VEGF and TNF-α between symptomatic and asymptomatic groups. Conclusions: Saliva can be a used as a substitute for serum to evaluate levels of the assessed biomarkers.

References

1. Gupta S, Jawanda MK. Oral lichen planus: an update on etiology, pathogenesis, clinical presentation, diagnosis and management. Indian J Dermatol. 2015;60(3):222.
2. Kurago ZB. Etiology and pathogenesis of oral lichen planus: an overview. Oral Surg Oral Med Oral Pathol Oral Radiol. 2016;122(1):72-80.
3. Ahmed I, Nasreen S, Jehangir U, Wahid Z. Frequency of oral lichen planus in patients with noninsulin dependent diabetes mellitus. J Pak Assoc Derma. 2017;22(1):30-4.
4. Nogueira PA, Carneiro S, Ramos‐e‐Silva M. Oral lichen planus: an update on its pathogenesis. Int J Dermatol. 2015;54(9):1005-10.
5. Akhurst RJ, Hata A. Targeting the TGFβ signalling pathway in disease. Nat Rev Drug Discov. 2012;11(10):790-811.
6. Lu R, Zhang J, Sun W, Du G, Zhou G. Inflammation‐related cytokines in oral lichen planus: An overview. J Oral Pathol Med. 2015;44(1):1-14.
7. Saravi ZZ, Seyedmajidi M, Sharbatdaran M, Bijani A, Mozaffari F, Aminishakib P. VEGFR-3 Expression in Oral Lichen Planus. APJCP. 2017;18(2):381.
8. Metwaly H, Ebrahem MA-M, Saku T. Vascular endothelial growth factor (VEGF) and inducible nitric oxide synthase (iNOS) in oral lichen planus: An immunohistochemical study for the correlation between vascular and inflammatory reactions. JOMSDA. 2014;26(3):390-6.
9. Sugerman P, Savage N, Walsh L, Zhao Z, Zhou X, Khan A, et al. The pathogenesis of oral lichen planus. Crit Rev Oral Biol Med. 2002;13(4):350-65.
10. Cheng Y-SL, Gould A, Kurago Z, Fantasia J, Muller S. Diagnosis of oral lichen planus: a position paper of the American Academy of Oral and Maxillofacial Pathology. Oral Surg Oral Med Oral Pathol Oral Radiol. 2016;122(3):332-54.
11. Dudhia BB, Dudhia SB, Patel PS, Jani YV. Oral lichen planus to oral lichenoid lesions: Evolution or revolution. JOMFP. 2015;19(3):364.
12. Drogoszewska B, Chomik P, Polcyn A, Michcik A. Clinical diagnosis of oral erosive lichen planus by direct oral microscopy. AAdv Dermatol Allergol. 2014;31(4):222.
13. Kistenev YV, Borisov AV, Titarenko MA, Baydik OD, Shapovalov AV. Diagnosis of oral lichen planus from analysis of saliva samples using terahertz time-domain spectroscopy and chemometrics. J Biomed Opt. 2018;23(4):045001.
14. Pezelj-Ribaric S, Prpic J, Glazar I. Saliva as a diagnostic fluid. Sanamed. 2015;10(3):215-20.
15. Kaur J, Jacobs R. Proinflammatory cytokine levels in oral lichen planus, oral leukoplakia, and oral submucous fibrosis. J Korean Assoc Oral Maxillofac Surg. 2015;41(4):171-5.
16. Agha-Hosseini F, Mirzaii-Dizgah I, Mohebbian M, Sarookani M-R. Vascular Endothelial Growth Factor in Serum and Saliva of Oral Lichen Planus and Oral Squamous Cell Carcinoma Patients. J Kerman Univ Medical Sci. 2018;25(1):27-33.
17. Malathi N, Mythili S, Vasanthi HR. Salivary diagnostics: a brief review. ISRN dentistry. 2014;2014.
18. Arunkumar S, Arunkumar J, Krishna N, Shakunthala G. Developments in diagnostic applications of saliva in oral and systemic diseases-A comprehensive review. J Sci Innov Res. 2014;3(3):372-87.
19. Zhou ZT, Wei BJ, Shi P. Osteopontin expression in oral lichen planus. J Oral Pathol Med. 2008;37(2):94-8.
20. Nafarzadeh S, Ejtehadi S, Shakib PA, Fereidooni M, Bijani A. Comparative study of expression of smad3 in oral lichen planus and normal oral mucosa. IJMCM. 2013;2(4):194.
21. Zenouz AT, Pouralibaba F, Babaloo Z, Mehdipour M, Jamali Z. Evaluation of Serum TNF-α and TGF-β in Patients with Oral Lichen Planus. J Dent Res Dent Clin Dent . 2012;6(4):143-7.
22. Firth F, Friedlander L, Parachuru V, Kardos T, Seymour GJ, Rich A. Regulation of immune cells in oral lichen planus. Arch Dermatol Res. 2015;307(4):333-9.
23. Sinon SH, Rich AM, Parachuru VP, Firth FA, Milne T, Seymour GJ. Downregulation of toll‐like receptor‐mediated signalling pathways in oral lichen planus. J Oral Pathol Med. 2016;45(1):28-34.
24. Salvador B, Arranz A, Francisco S, Córdoba L, Punzón C, Llamas MÁ, Fresno M. Modulation of endothelial function by Toll like receptors. Pharmacol Res. 2016;108:46-56.
25. Vassilakopoulou M, Psyrri A, Argiris A. Targeting angiogenesis in head and neck cancer. Oral oncology. 2015;51(5):409-15.
26. Mardani M, Ghabanchi J, Fattahi MJ, Tadbir AA. Serum level of vascular endothelial growth factor in patients with different clinical subtypes of oral lichen planus. IJMS. 2012;37(4):233.
27. Domingues R, de Carvalho GC, Aoki V, da Silva Duarte AJ, Sato MN. Activation of myeloid dendritic cells, effector cells and regulatory T cells in lichen planus. J Transl Med. 2016;14(1):171.
28. Mozaffari HR, Ramezani M, Mahmoudiahmadabadi M, Omidpanah N, Sadeghi M. Salivary and serum levels of tumor necrosis factor-alpha in oral lichen planus: a systematic review and meta-analysis study. Oral Surg Oral Med Oral Pathol Oral Radiol. 2017;124(3):e183-e9.
29. Malarkodi T, Sathasivasubramanian S. Quantitative analysis of salivary TNF-α in oral lichen planus patients. Int J Dent. 2015;2015.
Published
2020-04-30
How to Cite
ABDELGHAFFAR, Amira Abdelwhab et al. Evaluation of serum and salivary transforming growth factor beta, vascular endothelial growth factor and tumor necrosis factor alpha in oral lichen planus.. Journal of Oral Research, [S.l.], v. 9, n. 2, p. 86-92, apr. 2020. ISSN 0719-2479. Available at: <http://joralres.com/index.php/JOR/article/view/joralres.2020.012>. Date accessed: 15 july 2020. doi: https://doi.org/10.17126/joralres.2020.012.
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Articles