Efficacy of copper sulphate on Candida albicans on heat-polymerized acrylic resin.

  • Elisa Huerta Patología y Diagnóstico Oral, Facultad de Odontología, Universidad de Valparaíso, Valparaíso, Chile.
  • Daisy Gorup Patología y Diagnóstico Oral, Facultad de Odontología, Universidad de Valparaíso, Valparaíso, Chile.
  • Manuel Manríquez Patología y Diagnóstico Oral, Facultad de Odontología, Universidad de Valparaíso, Valparaíso, Chile.
  • Rodrigo Cruz Choappa Laboratorio de Micología, Facultad de Medicina, Universidad de Valparaíso, Valparaíso, Chile.
  • Peggy Vieille Laboratorio de Micología, Facultad de Medicina, Universidad de Valparaíso, Valparaíso, Chile.
  • Wilfredo González-Arriagada Patología y Diagnóstico Oral, Facultad de Odontología, Universidad de Valparaíso, Valparaíso, Chile.|Centro de Investigación Interoperativo en Ciencias Odontológicas y Médicas (CICOM), Universidad de Valparaíso, Valparaíso, Chile.


The ageing of population is increasing, and a great percentage of these patients wear removable prostheses, and can suffer denture stomatitis, a condition that has been associated with candidiasis. Aims: To evaluate in vitro the effectiveness of Copper Sulfate against Candida albicans in samples of heat-polymerized acrylic resin, compared to nystatin, sodium hypochlorite and chlorhexidine.  Materials and Methods: Initially, the minimum inhibitory concentration (MIC) of copper sulfate for Candida albicans was determined by microdilution. Then, 54 resin samples were divided into 6 treatment groups corresponding to Nystatin 100.000 UI, Sodium Hypochlorite 0.5%, chlorhexidine 0.12%, Copper Sulfate 4.7µg/ml, Copper Sulfate 9.4µg/ml and physiological saline solution, in which samples were submerged for 6 hours. Resin samples were then washed and cultured on solid media at 37°C for 72 hours. The number of colony-forming units was determined using a Quebec colony counter. The statistical analysis was performed using the Kruskal-Wallis test and the Mann-Whitney U test.  Results: Copper sulfate at a concentration of 9.4μg/ml presented a similar effectiveness as the other control products regarding the reduction in the number of colonies of Candida albicans post-treatment. Conclusion: The effectiveness of copper sulfate against Candida albicans on acrylic resin was similar to that of nystatin, sodium hypochlorite and chlorhexidine.


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How to Cite
HUERTA, Elisa et al. Efficacy of copper sulphate on Candida albicans on heat-polymerized acrylic resin.. Journal of Oral Research, [S.l.], v. 9, n. 1, p. 57-62, mar. 2020. ISSN 0719-2479. Available at: <http://joralres.com/index.php/JOR/article/view/joralres.2020.009>. Date accessed: 30 oct. 2020. doi: https://doi.org/10.17126/joralres.2020.009.