Evaluation of the effect of coating Vertex denture lining ma terial with plant oils on microbial growth and hardness

  • Hala Khuder Ali Prosthetic Dept. College of Dentistry, University of Mosul, Iraq.
  • Eman Aziz Mustafa Dept of Dental Basic Sciences, Dentistry College, Mosul University, Iraq.
  • Marwah Marwan Shehab Prosthetic Dept. College of Dentistry, University of Mosul, Iraq.


Aims: To evaluate the effect of surface coating with natural plant oils (Salvia officinalis, ginger and eucalyptus) on Candida growth and the hardness of Vertex denture lining material. Materials and method: Forty five specimens were prepared from soft acrylic lining material, twenty five of which were 10x10x2mm in size for testing antifungal activity, and twenty samples were 20mm in diameter and 12mm in thickness, for testing shore A hardness after coating samples with three types of natural oils (Salvia officinalis, ginger and eucalyptus oils). Significant differences among the groups at (p≤0.05) level of significance were determined statistically with one way analysis of variance and Duncan's multiple range test Result:  Antifungal assay showed a significant difference between five groups regarding Candida albicans growth (p≤ 0.05). For the hardness test, comparing different times of storage in water (1, 7, 14, 30 days) revealed a significant difference within all groups (p≤0. 05). While comparing the groups coated with natural oils with the control group, significant differences were found between different times of storage in water (1, 7, 30 day) (p≤0.05), except at 14 days of water storage there was no significant difference between groups (p>0.05). Conclusion: All tested natural oils were effective as fungicidal agents and increased the softness and duration of soft acrylic lining material.


1. Hashem MI. Advances in soft denture liners: An Update. J Contemp Dent Pract. 2015;16(4):314-8.
2. Kreve S, Dos Reis AC. Denture liners: A Systematic Review relative to adhesion and mechanical properties. The scientific world journal. 2019 ,1-11.
3. Aghazadeh M, Bialvaei AZ, Aghazadeh M, Kabiri F, Saliani N, Yousefi M, Eslami H, kafil HS. Survey of the Antibiofilm and Antimicrobial Effects of Zingiber officinale (in Vitro Study). Jundishapur J Microbiol. 2016; 9(2): e30167.
4. Agarwal V, Lal P, Pruthi V. Effect of Plant Oils on Candida albicans. J Microbiol Immunol Infect. 2010;43(5):447–51.
5. Ghorbani A, Esmaeilizadeh M. Pharmacological properties of Salvia officinalis and its components. J Tradit Complement Med. 2017;7(4):433-40.
6. Al-Irhayim RN. Antifungal Action of Some Natural Oils on Acrylic Resin Denture Base Materials. Al-Rafidain Dent J. 2012;16 :295-300
7. Cruickshank R, Duguid JP, Marmion BP. Medical microbiology: The practice of medical microbiology. 12th Ed. Churchill Living stone. England. 1975.
8. Mese A, Guzel K. Effect of storage duration on the hardness and tensile bond strength of silicone and acrylic resin based resilient denture. J Prosthet Dent. 2008; 99:153-9.
9. Pahuja RK, Garg S, Bansal S, Dang RH. Effect of denture cleansers on surface hardness of resilient denture liners at various time intervals- an in vitro study. J Adv Prosthodont. 2013; 5(3): 270-7.
10. Francis K, Mamle O, Mante M, Vicki C. In vitro changes in hardness of sealed resilient lining materials on immersion in various fluids. J of Prosthodontics.2008; 17:384-91.
How to Cite
ALI, Hala Khuder; MUSTAFA, Eman Aziz; SHEHAB, Marwah Marwan. Evaluation of the effect of coating Vertex denture lining ma terial with plant oils on microbial growth and hardness. Journal of Oral Research, [S.l.], p. 40-43, aug. 2019. ISSN 0719-2479. Available at: <http://joralres.com/index.php/JOR/article/view/835>. Date accessed: 17 aug. 2019. doi: https://doi.org/10.17126/jor.v1i1.835.